| 摘要: |
| 采用ISSR分子标记检测海南岛青梅(Vatica mangachapoi Blanco)7 个自然居群192 株个体的遗传多样性及遗传结构。筛选的10 条ISSR 引物共扩增出清晰位点132 个，其中多态性位点130 个，多态性百分率(PPB)达98.48%。物种水平上，Nei's 基因多样性指数(H)为0.3391，Shannon多态性信息指数(I)为0.5095，总基因多样度(Ht)为0.3441，反映了很高的遗传多样性。AMOVA 分析显示，青梅的遗传变异主要存在于居群内个体间，但亦产生了相当程度的居群间遗传分化（Gst为0.332），另外 Mantel 检测表明，青梅的遗传距离与地理距离间没有显著的相关性(r<0.2)，地理隔离对青梅各自然居群间的基因流影响不明显。 |
| 关键词: 青梅 ISSR 遗传多样性 遗传分化 基因流 |
| DOI： |
| 分类号: |
| 基金项目:科技部科技基础平台项目“野生植物资源保护与可持续利用”子项目(2003DIA3N024)和科技部、国家林业局海南尖峰岭国家级森林生态系统定位研究站基础条件平台支撑项目的一部分研究内容。 |
|
| Genetic Diversity of Vatica mangachapoi in Hainan Island Marked by ISSR |
|
huangfeiben
|
| Abstract: |
| Genetic variation and structure of seven populations of Vatica mangachapoi in Hainan Island were investigated by Inter-simple sequence repeats (ISSR) method. Ten ISSR primers were used to generate 132 unambiguous loci, 130 of which were polymorphic and the percentage of polymorphic loci was 98.48%. A high level of genetic diversity was found in V. mangachapoi at the species level (Nei’s genetic diversity=0.3391,Shannon information index=0.5095, Total genetic diversity= 0.3441). The Nei’s coefficient differentiations (Gst) by ISSR was 0.3329,which showed that the main genetic
variation of V. mangachapoi accured within populations, while a moderate level of differentiation occured among populations. According to Mantel test analysis，there is no significant correlation between genetic distance and geographic distance(r<0.2). Therefore, it might imply that geographic isolation had very limited influence on the gene flow among populations. |
| Key words: Vatica mangachapoi ISSR genetic diversity genetic differentiation gene flow |
