| 摘要: |
| 对广宁红花油茶组织培养快繁育苗方法进行试验,结果表明,采用两种浓度升汞液(0.025%和0.1%)进行二次消毒的处理方法可将外植体污染率控制在50%以下。在快繁增殖阶段,培养基组分为WPM+TDZ4.0mg/L+6-BA1.0mg/L+NAA0.3mg/L+蔗糖30g/L可有效诱导外植体分化丛生芽；WPM+TDZ1.0 mg/L +6-BA1.0 mg/L +NAA0.3 mg/L+蔗糖30g/L可维持芽丛持续快速增殖；而WPM+6-BA1.5 mg/L +NAA0.5 mg/L+蔗糖30g/L有利于促进不定芽个体生长。通常的培养方法无法诱导植株生根,但经过针对调节极性表现的程序系列培养可显著提高植株极性反应水平,调整生理反应的同步性,促使不定根分化,生根率最高达90%。再生植株移栽的平均成活率为85.4%。 |
| 关键词: 广宁红花油茶 组织培养 快速繁殖 |
| DOI： |
| 投稿时间：2015-04-10修订日期：2015-05-05 |
| 基金项目: |
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| Tissue Culture and Rapid Propagation of Camellia semiserrata |
| Gong Zheng,Wang Hongfeng,Zhang Hong,HE Chunmei,XU Qiaolin |
| (Guangdong Provincial Key Laboratory of Bio-control for the Forest Disease and Pest;Guangdong Academy of Forestry) |
| Abstract: |
| This paper deals with the preliminary trial on tissue culture and rapid propagation of Camellia semiserrata. The results showed that the contamination rate of explants can be controlled below 50% through the method of twice disinfection using mercuric chloride (0.025% and 0.1%). According to the results , the optimum medium for explants induction was WPM TDZ4.0mg/L 6-BA1.0mg/L NAA0.3mg/L sucrose30g/L; the optimum medium for bud fast multiplication was WPM TDZ1.0 mg/L 6-BA1.0 mg/L NAA0.3mg/L sucrose30g/L; and the suitable medium for shoot growth was WPM 6-BA1.5 mg/L NAA0.5 mg/L sucrose30g/L. Though customary methods could not induce rooting,the shoots smoothly rooting by means of pattern culture aiming at physiological regulation and polarity express, and root formation frequency could reach 90%.The mean of plantlet survival rate reached 85.4%. |
| Key words: Camellia semiserrata tissue culture rapid propagation |
