| 摘要: |
| 以新选育的木荷优良家系为材料，开展组培快繁育苗技术研究。结果表明，木荷适宜的初代培养基为GD 6-BA 1.50mg?L-1 NAA 0.50 mg?L-1，继代培养基为GD 6-BA 1.00mg?L-1 IAA 0.30 mg?L-1，生根培养基为1/2GD IBA 1.00mg?L-1 NAA 0.05mg?L-1 IAA0.80mg?L-1。芽苗增殖倍数为3.1倍，生根率达96.67％。探索出适宜的驯化、移栽和后期管理技术，使优良家系生根苗的移栽存活率达到92.2％。 |
| 关键词: 木荷 优良家系 组织培养 |
| DOI： |
| 投稿时间：2015-03-23修订日期：2015-04-03 |
| 基金项目: |
|
| Tissue Culture and Micropropagation of Superior Families of Schima superba Gardn.et Champ |
| zhou lihua,,he boxiang |
| (Guangdong Provincial Key Laboratory of Bio-control for the Forest Disease and Pest, Guangdong Academy of Forestry, Guangzhou) |
| Abstract: |
| A newly-selected superior family of Schima superba Gardn.et Champ was used for tissue culture and micropropagation. The explants of stem segments displayed the optimum initial growth on the primary media GD 6-BA 1.50 mg?L-1 NAA 0.50 mg?L-1, the highest proliferation rate of 3.1 times on subculture GD 6-BA 1.00 mg?L-1 IAA 0.30 mg?L-1, and 96.67% rooting rate on medium 1/2GD IBA 1.00mg?L-1 NAA 0.05mg?L-1 IAA0.80mg?L-1 Suitable techniques and procedures were identified for acclimatization, transplantation and later-period management of tissue-cultured plantlets. The survival rate of the superior family was 92.2%. |
| Key words: Schima superba Gardn.et Champ, superior families, tissue culture |
