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3种真菌抗生素对绿僵菌和6种非目标真菌的生长影响分析
杨华,徐金柱,秦长生,揭育泽
广东省林业科学研究院,广东省林业科学研究院,广东省林业科学研究院,广东省林业科学研究院
摘要:
文章比较了两性霉素、伊曲康唑和放线菌酮对土壤内金龟子绿僵菌(Metarhizium anisopliae)、黄绿绿僵菌(M. flavoviride)和6种非目标真菌生长的影响。结果表明,伊曲康唑对毛霉(Mucor racemosus)和黑曲霉(Aspergillus niger)的抑菌效果较差,浓度达200 μg/mL时对毛霉的抑制率仅达54.84%,且对绿僵菌生长的抑制作用显著大于两性霉素和放线菌酮(P<0.01),不适宜用于土壤绿僵菌的分离。两性霉素和放线菌酮在浓度为100 μg/mL时,对6种非目标真菌抑制率达69%以上,可作为绿僵菌分离的真菌抗生素。
关键词:  两性霉素  伊曲康唑  放线菌酮  抑菌  绿僵菌
DOI:
分类号:S763.15
基金项目:广东省林业科技创新专项(2012KJCX016 01);广东省科技计划项目(2012A020602086)。
The Influence of 3 Anti fungal Agents on the Growth of Metarhizium and 6 Non target Fungis
YANG Hua,XU Jin-zhu,QIN Chang-sheng and JIE Yu-ze
Guangdong Academy of Forestry Science,,
Abstract:
The effects of 3 anti fungal agents on the growth of Metarhizium anisopliae, M. flavoviride strains and 6 non target fungis were studied. The results showed that itraconazole did not have good inhibitory effects on Mucor racemosus and Aspergillus niger, the rate of M. racemosus controlling was 54.84%,but the inhibitory effects on the growth of Metarhizium were significantly higher than that of amphotericin and cycloheximide (P<0.01), suggesting it was not suitable for Metarhizium isolation from soil. Amphotericin and cycloheximide of 100 μg/mL could inhibit over 69% of 6 non target fungis, and were the most suitable anti fungal agents for Metarhizium isolation.
Key words:  amphotericin  itraconazole  cycloheximide  inhibitory  Metarhizium