| 摘要: |
| 对铁皮石斛组织培养育苗和组培苗培植技术进行研究，结果表明：在MS培养基中添加6-BA 1.0 mg/L+NAA 0.1 mg/L+IAA 0.5 mg/L+50 g/L椰子汁有利于芽苗的增殖；在1/2MS培养基中添加6-BA 0.1 mg/L+IBA 1.0 mg/L+NAA 0.2 mg/L+50 g/L椰子汁有利于小苗生根和植株生长。搭建大棚移植铁皮石斛组培苗，采用高架苗床，选用松树皮和花生壳（v/v=4∶1）为基质，移栽成活率可达95％以上。 |
| 关键词: 铁皮石斛 组织培养 培植技术 |
| DOI： |
| 投稿时间：2014-03-02修订日期：2014-03-24 |
| 基金项目: |
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| Study on the Techniques of Tissue Culture and Cultivation of Dendrobium officinale |
| Luo Wanye,Luo Wanzhou,Wei Jinqiu,Chen Guiqiong,zhuxijiao |
| (Guangdong Province Meizhou City Forestry Science Institute,Guangdong Meizhou) |
| Abstract: |
| The tissue culture and cultivation techniques of Dendrobium officinale were studied, the results showed that: the MS medium supplemented with 6-BA 1.0 mg/L NAA 0.1 mg/L IAA 0.5 mg/L 50 g/L coconut juice was good for bud proliferation; the 1/2MS medium supplemented with 6-BA 0.1 mg/ L IBA 1.0 mg/L NAA 0.2 mg/L 50 g/L coconut juice was good for rooting and growth of seedlings.The transplantion survival rate could reach above 95%,when the plantlets of D. officinale were planted under the conditions of greenhouse, elevated seedbed and growing media with pine bark peanut shell (v/v =4:1). |
| Key words: Dendrobium officinale tissue culture cultivation techniques |
