| 摘要: |
| 通过24孔板测定了几种农药原药和乙醇对松材线虫的杀线活性。试验结果表明嘧啶氧磷杀线活性最高，它的致死中浓度LC50为32.65 μg/mL，95%的置信区间为25.08~42.51 μg/mL；体积分数为5%的乙醇在20 min内使94.4%的松材线虫麻痹，体积分数为10%的乙醇在90 s内将96.7%的线虫杀死。另外，松材线虫浸入供试药剂的时间越长，所测定的LC50的值越小，克百威、灭线磷、嘧啶氧磷的LC50值从24 h 到72 h分别由448.47，51.96，94.54 μg/mL降低至51.47，36.94，32.65 μg/mL。 |
| 关键词: 松材线虫 嘧啶氧磷 乙醇 生物活性测定 LC50 |
| DOI： |
| 分类号:S763.3 |
| 基金项目:高等学校博士学科点专项科研基金(20114404110020) |
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| Potent Nematicidal Activity of Pirimioxyphos against Bursaphelenchus xylophilus |
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HONG Sen,WANG Xin-rong and XU Han-hong
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Key Laboratory of Pesticide and Chemical Biology, South China Agricultural University
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| Abstract: |
| Several pesticides and ethanol were tested against Bursaphelenchus xylophilus using 24well plate in vitro. The results showed that the most active pesticide was pirimioxyphos with LC50 value of 32.65 μg/mL, and it′s 95% confidence limit was 25.08—42.51 μg/mL; B. xylophilus were paralyzed within 20 minutes after they were immersed in the 5% ethanol, and were killed within 90 seconds after they were immersed in 10% ethanol. In addition, the longer the immersion time of the test agent against B. xylophilus, the smaller the LC50 value was, the LC50 values of carbofuran, ethoprophos, pirimioxyphos reduced from 24 to 72 h by 448.47,51.96,94.54 μg/mL to 51.47,36.94,32.65 μg/mL. |
| Key words: Bursaphelenchus xylophilus pirimioxyphos ethanol bioassay Lethal Concentration 50 |
