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| 广宁红花油茶根尖压片制作技术 |
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李永泉,陈清凤,张应中,丁晓纲,蔡坚,刘喻娟
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广东省林业科学研究院 广州 510520,广东省林业科学研究院 广州 510520,广东省林业科学研究院 广州 510520,广东省林业科学研究院 广州 510520,广东省林业科学研究院 广州 510520,广东省林业科学研究院 广州 510520
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| 摘要: |
| 广宁红花油茶在广东、广西等原生地种植容易获得高产，具有良好的遗传改良潜力。文章以广宁红花油茶为对象，探索了适用于荧光原位杂交技术中的染色体制片技术，并与传统的染色体压片制作技术进行比较，总结了一套节省时间、技术要求较低和效果较好的染色体根尖压片制作技术，可用于广宁红花油茶的细胞学研究，为开展分子染色体工程育种以及基因组分析奠定技术基础。 |
| 关键词: 染色体〓根尖〓压片技术〓荧光原位杂交 |
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| 基金项目:广东省自然科学基金博士启动项目“油茶多倍体育种技术研究”(No.10451052002005587)；广东省林业科技创新专项资金项目“油茶良种选育、无公害栽培技术研究与示范”(2011KJCX014 04)。 |
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| A Root Tip Squash Technique for Camellia semiserrata |
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Li Yongquan,Chen Qingfeng,Zhang Yingzhong,Ding Xiaogang,Cai Jian and Liu Yujuan
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Guangdong Academy of Forestry,Guangzhou,510520,Guangdong Academy of Forestry,Guangzhou,510520,Guangdong Academy of Forestry,Guangzhou,510520,Guangdong Academy of Forestry,Guangzhou,510520,Guangdong Academy of Forestry,Guangzhou,510520
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| Abstract: |
| It is easier to acquire high yield of Camellia semiserrata if they are cultivated in their natural distribution area, such as Guangdong and Guangxi province. The yield character had a well potential of genetic improvement. The article summarizes a root tip squash technique for C. semiserrata. This technique could be applied on fluorescence in situ hybridization (FISH) study and it is easy to carry out, time saving and has better effect than traditional technique. It could also be applied on cytological studies, chromosome engineering and analysis of plant genome as a basic technique. |
| Key words: chromosome, root tip, squash technique, FISH |
