| 摘要: |
| 利用发根农杆菌ATCC15834诱导金铁锁获得毛状根，研究金铁锁毛状根的最优培养条件。结果表明，在5种培养基中，以在1/2MS培养基中皂苷的总产量最大；添加3%蔗糖、水解乳蛋白(LH)及IBA利于毛状根的生长及总皂苷的积累；而添加IAA、NAA、6 BA及蛋白胨抑制其生长。 |
| 关键词: 金铁锁〓毛状根〓培养〓皂苷 |
| DOI： |
| 分类号: |
| 基金项目:林业公益性行业专项“华南立体林业经营模式研究与示范”（项目任务书编号：201004047）。 |
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| Effects of Culture Conditions on the Hairy Roots Growth \=of Psammosilene tunicoides |
|
Wang Hongfeng
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| Abstract: |
| Hairy root can be induced from Psammosilene tunicoides by Agrobacterium rhizogenes ATCC15834. The effects of different culture conditions on hairy roots growth were studied. Among five media used, the medium 1/2 MS was the best one for the saponins content. On the source concentration concerned, 3% sucrose concentration could increase the hairy root growth. The hydrolytic lactalbumin and IBA could improve the hairy root growth and total saponins production. IAA, NAA, 6 BA and peptone could inhibit hairy root growth. |
| Key words: Psammosilene tunicoides, hairy roots, culture, saponins |
