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应用电导法评价5个桉树抗寒性
裘珍飞1
中国林业科学研究院热带林业研究所
摘要:
文章从材料和条件控制上探讨电导法评价桉树抗寒性的应用,具体评价了4个桉树无性系和邓恩桉实生种的抗寒性。结果表明:EG 6无性系抗寒性较强,叶片半致死温度为-9.76~-8.95℃,井冈1号和广9次之,叶片半致死温度为-9.26~-8.22℃,邓恩桉和DH201 2较弱,叶片半致死温度为-8.80~-7.77℃。不同叶龄间:成熟叶比幼叶耐寒0.5~1.0℃ (除DH201 2为0.25℃外),老叶比成熟叶耐寒0~0.5℃。5个桉树在-3℃下冷冻24 h不造成严重冻害;在-6℃下冷冻2~4 h,细胞膜遭受严重损害。相对电导率随材料含水率的降低而降低,因此应用电导法测评抗寒性时应避免含水率增减对结果的影响。
关键词:  桉树〓电导率〓抗寒性〓含水率
DOI:
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基金项目:国家863项目“白桦、桉树等分子育种与品种创制”(2011AA100202)和中央科研院所基础专项“桉树转基因育种”(2007 23)。
The Cold Resistance Evaluation of 5 Eucalyptus Clones or \=Species by Conductivity Measurement
Qiu Zhen-Fei
Abstract:
This paper conducted a study of Eucalyptus cold resistance by the conductivity measurement of different plant materials under different conditions. The cold resistance of four Eucalyptus clones and E. dunnii was evaluated. Clone EG 6 had the strongest cold resistance, the lethal temperatures of leaves were -9.76~ -8.95℃. The following clones were Jinggang 1 and Guang 9, the lethal temperatures of leaves were -9.26~-8.22℃. E. dunnii and clone DH201 2 have lower cold resistance, the lethal temperatures of leaves were -8.80~ -7.77℃. There was a law of cold resistance in different leaf age: Cold resistance temperature of mature leaf was enhanced 0.5~ 1.0℃ as compared with that of young leaf, but clone DH201 2 was 0.25℃, old leaf cold temperature was enhanced 0~ 0.5℃ as mature leaf. In tested clones and species, No serious physiological injury were occurred at -3℃ lasting 24 h, but the cell membrane were severely damaged at -6℃ lasting 2~4 h. The relative conductivity declined when the water content of the materials decreased. Hence, water content changes of the materials should be avoided when the relative conductivity method was adopted to evaluate cold resistance.
Key words:  Eucalyptus, conductivity, cold resistance, water content