| 摘要: |
| 大型真菌中的一些类群的子实体由于含胶质和多糖较多，对提取高质量的DNA及随后的分子生物学实验有很大影响。本文针对这一现象，对过去大型真菌的DNA提取方法进行了改进，提出一种实用、高效的DNA提取方法。并通过实验证明，在大型真菌DNA提取过程中，此方法对去除样品中胶质和多糖的效果明显，可有效获得高质量的DNA，满足随后进行的分子生物学研究需要。 |
| 关键词: 大型真菌 DNA 提取方法 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目（NO.31070014）；中国林业科学研究院热带林业研究所基本科研业务费专项资助项目（RITFKYYW2010 10）。 |
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| An IMPROVED PROTOCOL FOR EXTRACTION OF DNA FROM MACROFUNGI |
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Zhou Lingling, Liang Junfeng
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Research Institute of Tropical Forestry, Chinese Academy of Forestry
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| Abstract: |
| Some macrofungi contain colloids and polysaccharides, which have a negative effect on extraction of high-quality DNA and subsequent molecular biological experiments. In order to reduce the negative effect, a practical and efficient protocol for extraction of macrofungi DNA was improved. It showed that the improvement could reduce colloids and polysaccharides of isolating DNA from the higher fungi effectively, which was beneficial for carrying out the subsequent molecular biological studies. |
| Key words: macrofungi DNA isolating methods |
