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寄生加勒比松松材线虫的鉴定及其致病性测定*
方天松1
广东省森林病虫害防治与检疫总站
摘要:
运用线虫比较形态学和rDNA-ITS-RFLP方法对采自广东省广州市白云山加勒比松上的伞滑刃线虫进行鉴定,结果确定该线虫为松材线虫。致病性测定表明该松材线虫种群对加勒比松苗有较强的致病力,接种的松苗均出现不同程度的枯萎,而且从枯死木中可重新分离到该线虫。
关键词:  松材线虫 分子鉴定 加勒比松 致病性
DOI:
分类号:
基金项目:本文由省科技计划项目“2004B20501005”资助
Identification of Bursaphelenchus xylophilus from Pinus caribaea and pathogenicity assay
fangtiansong
Abstract:
Bursaphelenchus xylophilus collected from Pinus caribaea in Guangzhou city, Guangdong province using morphology and rDNA-ITS-RFLP were confirmed. Seedlings of P. caribaea were inoculated with this population. B. xylophilus displayed pathogenicity in the host seedlings. All seedlings inoculated with pine wood nematodes wilted. In additon, B. xylophilus were recovered from the seedling.
Key words:  Bursaphelenchus xylophilus, molecular identification, Pinus caribaea, pathogenicity